产品货号：
Z28245
中文名称：
TOP2A-Specific抗体
英文名称：
Anti-TOP2A-Specific Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-TOP2A-Specific Antibody |
| 指标别名 | DNA topoisomerase 2 alpha;TOP2;TOP2A;TOP2A-Specific;TP2A |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,ICC/IF,FCM |
| 基因名称 | TOP2A |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence of human Topoisomerase II alpha/TOP2A (EDYLYGQTTTYLTYND). |
| 实际分子量 | 174KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22.The TOP2A gene spans approximately 30kb and contains 35 exons.Furthermore,DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes.Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules,catenation,decatenation,knotting,and unknotting of circular DNA.It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments.There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus,constituting about 0.1% of the nuclear extract1.DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation.Specific inhibitors of topoisomerase II blocked transcription on chromatin templates,but did not affect transcription on naked templates.Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II2. |
| Uniprot ID | P11388 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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